Making E. coli an Erythromycin Production Plant
نویسندگان
چکیده
منابع مشابه
Production of an E. coli Toxin Protein; Colicin A in E. coli Using an Inducible System
The ultimate aim of biochemistry is to decipher how events occur inside of living cells or organisms. The living cell must also be regarded as the ideal model system for any biochemical application, as most physiological targets are found inside living cells. The cell envelope of the Gram-negative bacterium Escherichia coli is a complex structure and many of the proteins found in the bacterial ...
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Since the discovery of erythromycin A (1; Fig. 1)1} more than 25 biosynthetic representatives of this family of 14-membered macrolide antibiotics have been reported. Most of erythromycins are produced by the genera Saccharopolyspora and Streptomyces but few by Nocardia strains2). Erythromycin E (2; Fig. 1) is distinguishable from all the other known macrolide antibiotics and other microbial met...
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A novel strategy to increase protein expression yield is unintended induction of expression in complex media, called auto-induction. This method can be used to express proteins under control of the lac promoter without any need to monitor bacterial growth pattern, and addition of specific expression inducers such as Isopropyl β-D-1-thiogalactopyranoside (IPTG) at proper time. In the present stu...
متن کاملHigh-yield Production of Granulocyte-macrophage Colony-stimulating Factor in E. coli BL21 (DE3) By an Auto-induction Strategy
A novel strategy to increase protein expression yield is unintended induction of expression in complex media, called auto-induction. This method can be used to express proteins under control of the lac promoter without any need to monitor bacterial growth pattern, and addition of specific expression inducers such as Isopropyl β-D-1-thiogalactopyranoside (IPTG) at proper time. In the present stu...
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This paper concerns a precise study on the nature of the binding of erythromycin to Escherichia coli (Q13) ribosomes. (1) An equimolar amount of erythromycin bound to 50S ribosomal subunits at extremely low concentrations and this binding was not affected by the addition of 30S ribosomal subunits. (2) The binding of erythromycin to ribosomes was not significantly influenced by decreasing the Mg...
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ژورنال
عنوان ژورنال: Chemistry & Biology
سال: 2010
ISSN: 1074-5521
DOI: 10.1016/j.chembiol.2010.11.002